Ozone Exposure
Ozone Exposure - Related Videos
08:17
Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure
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Cited by 7 •
2017
This study describes the successful generation of a new chronic obstructive pulmonary disease (COPD) animal model by repeatedly exposing mice to high concentrations of ozone.
07:49
Measuring Tropospheric Ozone
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Ozone is a form of elemental oxygen (O3), a molecule of three oxygen atoms bonded in a structure that is highly reactive as an oxidizing agent. Ozone occurs in both the stratosphere and the troposphere levels of the atmosphere. When in the stratosphere (located approximately 10-50 km from the earth’s surface), ozone molecules form to the ozone layer and help prevent harmful UV rays from reaching Earth’s surface. In...
08:54
In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
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Cited by 15 •
2019
This manuscript describes a protocol for determining whether exposure to ozone, a criteria air pollutant, impairs alveolar macrophage efferocytosis in vivo. This protocol utilizes commonly used reagents and techniques and can be adapted to multiple models of pulmonary injury to determine effects on alveolar macrophage efferocytosis.
09:08
Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
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Cited by 11 •
2016
In the current climate of scarce resources, new technologies are emerging that allow researchers to conduct studies cheaper, faster and with more precision. Here we describe the development of a bead-based salivary antibody multiplex immunoassay to measure human exposure to multiple environmental pathogens simultaneously.
07:07
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
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Cited by 9 •
2019
Here we describe a method to assess lung expression of miRNAs that are predicted to regulate inflammatory genes using mice exposed to ozone or filtered air at different stages of the estrous cycle.
FAQs
Ozone reacts directly with airway-lining fluids, lipids, and proteins after inhalation. These reactions generate oxidative stress, which can activate inflammatory signaling rather than acting only as a physical irritant. The resulting biochemical changes provide a mechanistic link between polluted air exposure and later effects on respiratory and immune function.
Changes to the airway epithelial barrier can influence how effectively the respiratory tract maintains its protective interface. Because ozone may alter this barrier while also affecting innate immune responses, exposure can change antimicrobial defenses at multiple levels. Immunology studies therefore examine epithelial effects alongside inflammation when evaluating infection-related outcomes.
Ozone exposure may affect susceptibility to respiratory pathogens, disease severity, and recovery by changing inflammatory signaling, epithelial integrity, innate immune activity, and antimicrobial defenses. These outcomes are connected but not identical: an exposure could alter the initial defense response, the intensity of disease-associated inflammation, or the ability to recover after infection.
Antioxidant and anti-inflammatory strategies are investigated because ozone-related harm includes oxidative stress and activated inflammatory signaling. Antioxidant approaches target the oxidative component, whereas anti-inflammatory approaches address downstream immune activation. Studying both types helps researchers determine which biological processes contribute most strongly to altered respiratory or infection-related outcomes.
Controlled exposure models provide a defined way to examine how ozone affects respiratory and immune responses under experimental conditions. Researchers can relate the exposure to changes in epithelial barriers, inflammatory signaling, innate immunity, or antimicrobial defenses, then compare those findings with infection-related outcomes. This design helps separate ozone-associated effects from broader pollution conditions.
Relevant outcomes include changes in respiratory function, epithelial barrier status, inflammatory signaling, innate immune responses, and antimicrobial defenses. In infection-focused work, investigators can also examine whether these changes correspond with altered susceptibility, more severe disease, or differences in recovery. Considering several endpoints together clarifies how airway injury and immune modulation may interact.
Ozone exposure research connects mechanistic immunology with population protection because it examines how polluted air may influence respiratory health and responses to pathogens. Findings from controlled models can support evaluation of public-health standards and guide investigation of strategies intended to reduce ozone-related harm, including antioxidant or anti-inflammatory approaches.