Pseudorabies Virus Model
Pseudorabies Virus Model - Related Videos
02:00
Generating Virus-Induced Neuroinflammation in a Mouse Model
0 Views •
2025
This video demonstrates the generation of neuroinflammation in a mouse model. An anesthetized mouse with micro-wounds is taken, and an inoculum of pseudorabies virus (PRV) is applied to the wounds. The virus infects the peripheral nerves, spreads to the dorsal root ganglia (DRG), and further infects the spinal cord and brain. Infection of neurons in the nervous system leads to an immune response that induces neuronal damage, leading to neuroinflammation.
13:12
Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
0 Views •
Cited by 9 •
2015
Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.
10:11
Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
0 Views •
Cited by 54 •
2014
Work with infectious Ebola viruses is restricted to biosafety level 4 laboratories. Tetracistronic minigenome-containing replication and transcription-competent virus like particles (trVLPs) represent a lifecycle modeling system that allows us to safely model multiple infectious cycles under biosafety level 2 conditions, relying exclusively on Ebola virus components.
10:44
Influenza A Virus Studies in a Mouse Model of Infection
0 Views •
Cited by 38 •
2017
Influenza A viruses (IAVs) are important human respiratory pathogens. To understand the pathogenicity of IAVs and to perform preclinical testing of novel vaccine approaches, animal models mimicking human physiology are required. Here, we describe techniques to evaluate IAV pathogenesis, humoral responses and vaccine efficacy using a mouse model of infection.
00:50
What are Viruses?
0 Views •
2019
Overview A virus is a microscopic infectious particle that consists of an RNA or DNA genome enclosed in a protein shell. It is not able to reproduce on its own: it can only make more viruses by entering a cell and using its cellular machinery. When a virus infects a host cell, it removes its protein coat and directs the host’s machinery to transcribe and translate its genetic material. The hijacked cell assembles the replicated components into thousands of viral progeny, which can rupture and...
FAQs
After initial infection in peripheral tissues, pseudorabies virus can move backward along axons toward the nervous system. This route connects events at the original infection site with neuronal involvement and allows investigators to examine how viral spread reaches neural tissue. Studying this progression helps clarify the relationship between peripheral infection, neuroinvasion, and nervous-system disease.
The possible establishment of latent infection means that viral effects may extend beyond the initial phase of active spread. Investigators can therefore consider how the virus persists in relation to neurons, host immunity, and later disease processes. This feature makes the model useful for examining herpesvirus biology and the long-term consequences of nervous-system involvement.
Host defenses can shape viral spread, inflammation, antiviral responses, and the severity of disease. The model allows researchers to examine these responses as connected processes rather than isolated outcomes, including how immunity interacts with infection in peripheral tissues and the nervous system. Such analysis can identify host factors associated with more or less severe disease.
A study can follow the infection from entry into peripheral tissues through movement along axons and interaction with the nervous system. Researchers may then assess viral spread, inflammation, antiviral responses, and possible latent infection. Organizing observations across these stages helps distinguish local infection from neuroinvasion and clarifies how host responses change during disease progression.
These models are useful when an intervention must be considered in the context of both viral infection and nervous-system involvement. Vaccine or antiviral studies can examine whether treatment influences pathogen spread, inflammation, antiviral responses, or disease severity. The resulting information supports evaluation of strategies aimed at limiting infection and its consequences.
The model connects virology with immunology and neuroinfectious disease by allowing investigators to study pathogen movement, neuronal involvement, inflammation, and host defense together. It can provide context for understanding herpesvirus biology, identifying host factors that affect outcomes, and examining why infection becomes more severe when the nervous system is involved.