Siv-specific Cd8+ T Cells
Siv-specific Cd8+ T Cells - Related Videos
04:25
A Peptide-MHC-I Tetramer Staining Technique to Analyze SIV-Specific CD8+ Memory T Cells
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2025
This video demonstrates a method for characterizing CD8+ memory T cells specific to simian immunodeficiency virus (SIV) using peptide-major histocompatibility complex class I (pMHC-I) tetramers. A pMHC-I tetramer is a complex of four MHC-I molecules bound to SIV-specific peptides and conjugated to a fluorophore. These tetramers selectively bind to T cell receptors (TCRs) on SIV-specific CD8+ T cells, facilitating the characterization of these cells.
06:20
An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells
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2025
This video demonstrates an assay to analyze antigen-specific CD8+ T cells in different cell cycle stages. Fluorescently-labeled viable cells are selected using flow cytometry, and the cells in the different cell cycle stages are identified by their DNA content using antibodies against cell proliferation-associated nuclear protein and a nucleic acid stain.
05:41
Nanoparticle-Based Enrichment and Expansion of Antigen-Specific CD8+ T Cells
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2025
This video describes a protocol for isolating rare-antigen-specific CD8+ T cells using magnetic nanoparticle-based artificial antigen-presenting cells (aAPCs) functionalized with antigenic peptide-loaded MHC-Igs and anti-CD28 antibodies. Interaction of the aAPCs with CD8+ T cells specifically expressing the antigenic peptide-targeting T cell receptors, followed by their expansion in vitro, results in the enrichment and expansion of this CD8+ T cell population.
08:37
In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues
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Cited by 11 •
2017
Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.
20:47
A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
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Cited by 10 •
2009
A technique combining in situ tetramer staining and in situ hybridization (ISTH) enables visualization, mapping and analysis of the spatial proximity of virus-specific CD8+ T cells to their virus-infected targets, and determination of the quantitative relationships between these immune effectors and targets to infection outcomes.
FAQs
Activation depends on the T-cell receptor recognizing an SIV-derived peptide displayed by a major histocompatibility complex class I molecule on an infected cell. This recognition directs the response toward that target rather than producing a generalized reaction. The activated lymphocyte can then combine direct target-cell destruction with cytokine production, linking specificity to antiviral control.
Cytotoxic activity removes infected cells through the release of perforin and granzymes, molecules that support destruction of the recognized target. Cytokine production provides a separate antiviral function by suppressing viral replication. Because these outputs are mechanistically distinct, evaluating both helps determine whether a response is primarily destructive, suppressive, or capable of contributing through both pathways.
A high frequency indicates how many SIV-specific CD8+ T cells are present, but it does not by itself show whether they can destroy infected cells or produce cytokines. For that reason, researchers assess frequency together with functionality and antiviral activity. This multidimensional view distinguishes the size of a response from its effective contribution to immune control.
Characterization focuses on three related but distinct outcomes: the frequency of cells responding to SIV, the functions they can perform, and their antiviral activity. Considering these measurements together provides a more informative profile than any single readout. The resulting profile can help researchers compare immune responses, assess their effectiveness, and relate them to infection outcomes.
In nonhuman primate models, measurements of SIV-specific CD8+ T-cell frequency, functionality, and antiviral activity can be compared with patterns of immune control and disease progression. These relationships help researchers examine whether stronger or more effective cellular responses accompany better control of infection. The cells therefore serve as indicators for studying how antiviral immunity changes during disease.
Vaccine studies can examine whether immunization generates SIV-specific CD8+ T cells and determine the quality of those responses through their frequency, functionality, and antiviral activity. This approach goes beyond asking whether cells are present. It helps reveal whether vaccination promotes cellular immunity with properties relevant to suppressing viral replication and controlling infected cells.
These cells provide a nonhuman primate model for investigating principles of HIV-specific cellular immunity. Researchers can use the SIV system to study how peptide recognition, target-cell destruction, cytokine production, and antiviral activity relate to immune control. Findings from this model support the development of strategies intended to improve protective cellular responses against related viral infection.