Surface Marker Staining
Surface Marker Staining - Related Videos
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
03:20
Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells
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2025
This video demonstrates a protocol for co-culturing mouse brain endothelial cells and primary cortical stem cells and labeling the cell surface glycoproteins using Ac4ManNAz (azidosugar per-O-acetylated N-azidoacetylmannosamine). This method allows for selective enrichment and identification of cell surface proteins in primary cells.
09:38
FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
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Cited by 38 •
2011
Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
11:39
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
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Cited by 1 •
2019
Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.
FAQs
The information depends on whether each antibody recognizes the intended surface molecule. A fluorescent label converts that binding event into a detectable signal, so the selected antibodies determine which cell populations can be distinguished and compared. In immunology studies, marker choices can therefore reveal changes associated with immune-cell identity, activation, differentiation, or responses to pathogen exposure.
Flow cytometry detects and quantifies fluorescent signals from stained cells, making it useful for measuring marker-defined populations across a sample. Fluorescence microscopy instead visualizes labeled cells directly, preserving information about their appearance and location within the observed material. The choice depends on whether the study emphasizes population-level quantification or visual examination of individual stained cells.
A marker panel combines several antibodies so researchers can evaluate multiple surface features at once. This supports more detailed separation of cell populations than relying on one signal alone and helps relate marker combinations to activation or differentiation states. In infection research, panels can also reveal how several immune-cell characteristics change together after pathogen exposure.
Changes in detected marker patterns can indicate shifts in the composition or functional state of immune-cell populations. Comparing samples during pathogen exposure, or after an immune-targeted intervention, may show altered activation, differentiation, or population abundance. These observations help connect measurable cell-surface changes with broader questions about host responses and disease mechanisms.
The workflow begins by selecting antibodies against the surface molecules relevant to the research question. Those antibodies bind their matching targets on cells and provide fluorescent signals for detection. The stained sample is then examined by flow cytometry or fluorescence microscopy, allowing researchers to identify marker-defined populations, quantify signals, or visualize labeled cells.
This technique is useful when researchers need to characterize immune-cell populations or monitor how they change during pathogen exposure. It can support studies of host responses and disease mechanisms, while also allowing comparisons associated with vaccines or immune-targeted treatments. Its value comes from linking surface-marker patterns with measurable changes in immune-cell state.
Depending on the detection platform, results can include quantified fluorescent signals, the relative identification of marker-defined cell populations, or visual localization of labeled cells. These outputs support comparisons between experimental conditions and can reveal changes in immune-cell activation or differentiation. Such measurements may contribute to diagnostic pattern analysis and evaluation of treatment or vaccine effects.
Researchers can compare surface-marker patterns before and after vaccination or immune-targeted treatment to assess associated changes in immune-cell populations and functional state. The resulting flow-cytometry measurements or microscopy observations provide cellular evidence of altered host responses. These data can help evaluate whether an intervention is linked to changes in activation, differentiation, or disease-related immune patterns.