Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Qing-Ran Bai; Lu Dong; Qin Shen

doi:10.3791/58945

Journal

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Neuroscience

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Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

5,274 Views

•

11:39 min

•

September 07, 2019

DOI:

10.3791/58945-v

DOI:

10.3791/58945-v

•

11:39 min

September 07, 2019

•

5264 Views

Qing-Ran Bai*¹, Lu Dong*², Qin Shen¹

¹Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology,Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education ²College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University

Summary

Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.