Amylase Detection
Amylase Detection - Related Videos
03:26
Periodic Acid-Schiff Staining of Cells: An In Vitro Technique to Detect Glycogen Levels in Peripheral Blood Mononuclear Cells
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2025
This video demonstrates the periodic acid-Schiff staining technique for assessing glycogen levels of peripheral blood mononuclear cells in vitro. The stain imparts a magenta color to intracellular glycogen granules, rendering them visible under a microscope.
08:25
Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
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Cited by 20 •
2013
We demonstrate: (1) procedures for collection of salivary samples in cognitive impaired older adults by family caregivers in the home setting, (2) procedures for measuring stress activity via salivary cortisol and alpha amylase, and (3) representative profiles. Protocols that allow researchers to study stress-linked processes advance our understanding of biological sensitivity and susceptibility.
09:21
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
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Cited by 11 •
2019
Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein. Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
08:37
Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry
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Cited by 2 •
2012
Considering saliva sampling for future clinical application, a lollipop-like ultrafiltration (LLUF) probe was fabricated to fit in the human oral cavity. Direct analysis of undigested saliva by NanoLC-LTQ mass spectrometry demonstrated the ability of LLUF probes to remove large proteins and high abundance proteins, and make low-abundant peptides more detectable.
FAQs
Assays connect enzyme action to a measurable signal. A starch-based or synthetic substrate is exposed to the biological sample under controlled conditions, and amylase-driven hydrolysis is tracked through a change in color, turbidity, or substrate concentration. The selected readout translates the reaction into an estimate of enzymatic activity or, depending on assay design, amylase concentration.
These measurements describe related but different properties. Activity reflects how effectively the enzyme hydrolyzes the assay substrate under the specified conditions, whereas concentration estimates how much amylase is present. Consequently, the reported result depends not only on the biological sample but also on whether the assay is designed to quantify reaction performance or enzyme amount.
Reliable results require the reaction and specimen to be handled consistently. Assay design determines which substrate and signal are monitored, while controlled conditions help make measurements comparable. Sample handling can also affect the observed result, so inconsistent preparation or processing may reduce confidence in the reported amylase activity or concentration, even when the analytical principle is appropriate.
Typically, a biological sample is combined with a starch-based or synthetic substrate, and the reaction is maintained under controlled conditions. The assay then measures the resulting change in color, turbidity, or substrate concentration. That signal is used to report amylase activity or concentration. The exact workflow depends on the assay design, so readout selection is part of the procedure.
It is particularly useful when clinicians are evaluating pancreatic or salivary gland function. In suspected acute pancreatitis, the result can contribute to the laboratory assessment, but its meaning depends on the clinical situation. Symptoms, the timing of testing, and complementary laboratory findings should be considered together rather than treating the amylase result as an isolated conclusion.
Interpretation should account for the possibility that altered enzyme release can occur in more than one clinical context. Because amylase detection may reflect pancreatic or salivary gland activity, the result alone does not identify a single cause. Correlating it with symptoms, testing time, and complementary laboratory findings improves its diagnostic value and helps place the measurement in context.