Busin Injector
Busin Injector - Related Videos
13:36
Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
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Cited by 10 •
2010
Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.
05:06
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
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Cited by 10 •
2021
This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
10:05
Rodent Brain Microinjection to Study Molecular Substrates of Motivated Behavior
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Cited by 4 •
2015
Rodents are an appropriate model to investigate the molecular substrates of behavior and complex psychiatric disorders. Brain microinjection in awake rodents can be used to elucidate disease substrates. An efficient and customizable brain microinjection method as well as the execution of an operant paradigm that quantifies motivation is presented.
01:55
Intracranial Injection of Gut-Derived Microbial Metabolites in a Mouse Model
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2025
Source: Liou, C. et al. Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice. J. Vis. Exp. (2022).This video demonstrates the intracranial injection of short-chain fatty acids (SCFAs) in a freely moving mouse to study their effects on brain-mediated behavior. An injector cannula is inserted into a guide cannula implanted in the mouse brain. After recovery, the mouse acclimates in a novel cage. SCFAs are then administered, and locomotor behavior is recorded.
03:20
Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation
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2025
Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.
FAQs
The injector uses controlled fluid flow to move the prepared donor graft through a small corneal incision. This flow supports a measured insertion rather than requiring broad access to the eye, while the graft can unfold and be positioned against the posterior corneal surface. The mechanism therefore links delivery control with tissue placement and a less invasive surgical approach.
The Busin Injector's loading stage places the prepared donor graft within a device designed for controlled advancement. Once loaded, the surgeon can deliver the tissue through the incision and direct it toward the posterior corneal surface. This separates graft preparation from intraocular placement, supporting a more standardized insertion sequence and reducing the need for extensive direct manipulation.
Limiting surgical manipulation is important because the described approach aims to reduce handling while inserting donor tissue. The injector helps standardize how the graft enters the eye, and controlled delivery supports positioning at the posterior corneal surface. Together, these features are relevant when a procedure must replace diseased endothelial cells without removing much of the recipient cornea.
At a high level, the surgeon prepares donor corneal tissue, loads the graft into the injector, advances it through a small corneal incision using controlled fluid flow, and allows it to unfold against the posterior corneal surface. These stages connect tissue preparation, delivery, unfolding, and final positioning without requiring replacement of the recipient cornea as a whole.
The small incision provides the entry route for the loaded graft while supporting the minimally invasive character of endothelial keratoplasty. Because the tissue is advanced through this limited opening rather than introduced through broad corneal access, the injector must coordinate delivery with fluid flow and subsequent unfolding. The incision therefore connects device operation to preservation of much of the recipient cornea.
It may be considered when diseased corneal endothelial cells contribute to edema and reduced vision and endothelial keratoplasty is selected to replace those cells. In this setting, the instrument supports donor-tissue insertion while preserving much of the recipient cornea. Its practical value lies in combining a focused therapeutic target with controlled graft delivery and limited surgical manipulation.