Fitc Inulin Permeability Test
Fitc Inulin Permeability Test - Related Videos
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
02:45
Assessment of Intestinal Permeability in C. elegans Following Bacterial Exposure
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2026
Source: Le, T. A. N., et al., Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans. J. Vis. Exp. (2019)This video demonstrates a fluorescence-based assay to quantify intestinal permeability in C. elegans exposed to Pseudomonas aeruginosa. Compared to controls fed Escherichia coli, pathogen-treated worms show increased fluorophore leakage into the body cavity, indicating epithelial barrier damage.
07:22
Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease
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Cited by 15 •
2017
In this study, we present an easy and efficient procedure for evaluating the disruption of the blood-brain barrier under neurodegenerative conditions. To achieve our objective, we infused high molecular weight fluorescein isothiocyanate labelled (FITC)-albumin into mouse jugular vein and evaluated its leakage into the brain parenchyma by fluorescence microscopy.
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
FAQs
The assay converts tracer movement into a fluorescence signal that can be quantified in the compartment opposite the loading side. FITC labeling makes the inulin detectable, while the amount of fluorescence indicates how much tracer has crossed the biological model. This links a measurable optical readout to barrier integrity rather than relying only on visual inspection.
Greater passage generally indicates that the barrier has become more permeable, allowing more tracer to cross from the loaded side to the opposite compartment. In experimental medicine, this finding can support evidence of barrier disruption or reduced integrity. Lower passage supports the interpretation that the model restricts tracer movement more effectively under the tested condition.
The same permeability measurement can be performed with epithelial, endothelial, or other tissue models, but each represents a different biological barrier context. The model determines which barrier function is being examined and how the findings should be interpreted. Selecting an appropriate cell layer or tissue model helps connect tracer passage with the medical process under study.
A researcher first establishes the biological barrier model with separated compartments, then adds FITC-labeled inulin to one side. After allowing the experimental system to be assessed, fluorescence is measured in the opposite compartment to quantify tracer passage. Results are then compared across the relevant experimental conditions or treatment groups to evaluate differences in barrier function.
Each experimental condition can be evaluated by measuring the fluorescence associated with FITC-inulin that reaches the opposite compartment. Differences in signal provide a quantitative basis for comparing tracer passage between groups. A condition associated with greater fluorescence indicates higher permeability than a condition with less signal, supporting assessment of treatment-related changes in barrier integrity.
The method is useful when researchers need to examine barrier behavior in studies of inflammation, vascular dysfunction, drug transport, or disease-related disruption. Because it produces quantitative permeability measurements, investigators can compare how experimental conditions or treatments affect epithelial, endothelial, or other tissue barriers and relate those changes to the medical process being modeled.