Glomerulus Isolation
Glomerulus Isolation - Related Videos
03:18
Periodic Acid Schiff or PAS Staining: A Method to Assess the Structural Deformities of Glomerulus
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2023
In this video, we describe the protocol for performing Periodic Acid Schiff (PAS) stain to assess the structural anomalies in the kidney glomerulus. The PAS staining method enables appropriate evaluation of the glomerulus pathophysiology.
10:23
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
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Cited by 11 •
2022
Presented here is a protocol to engineer a personalized organ-on-a-chip system that recapitulates the structure and function of the kidney glomerular filtration barrier by integrating genetically matched epithelial and vascular endothelial cells differentiated from human induced pluripotent stem cells. This bioengineered system can advance kidney precision medicine and related applications.
03:35
Glomeruli Isolation: A Technique to Obtain Intact Glomeruli from Murine Kidney
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2023
This video describes the method to isolate intact glomeruli from mouse kidneys. The encapsulated and decapsulated glomeruli thus obtained can further be used for culturing parietal epithelial cell outgrowths from the murine kidney.
12:19
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Cited by 25 •
2015
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.
08:09
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
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Cited by 1 •
2017
We describe the isolation of cardiac extracellular matrix from C57Bl/6J control mice, tight-skin mice, and tight-skin mice treated with the IRF5 inhibitory peptide. We also describe the vasodilation studies on the isolated vessels from C57Bl/6J, tight-skin mice and tight-skin mice treated with the IRF5 inhibitory peptide.
FAQs
Separation depends on physical differences between glomeruli and the surrounding kidney tissue. Controlled dissociation releases the glomerular capillary tufts, while filtration, sieving, or density-based steps help retain these structures and remove smaller or differently buoyant tubules and connective tissue. The selected separation principle influences sample purity and the extent to which intact architecture is preserved for downstream analysis.
Mechanical dissociation must release glomeruli without excessive disruption of their structure. Preserving intact architecture is important because the capillary tuft can then be examined directly and analyzed in relation to cellular or molecular changes. If the tissue is not adequately dissociated, surrounding material may remain; if handling is too disruptive, structural information relevant to glomerular injury may be lost.
Both uses require separation from surrounding renal tissue, but the downstream objective differs. Microscopy benefits from maintaining recognizable glomerular architecture for visual examination, whereas molecular analysis focuses on measuring cellular or molecular changes within the isolated material. Consequently, the isolation workflow must balance structural preservation with sufficient sample preparation for the intended analytical method.
A typical workflow begins with controlled mechanical dissociation of kidney tissue. The resulting material then passes through filtration or sieving steps, or undergoes density-based separation, to distinguish glomeruli from tubules and connective tissue. The recovered structures can subsequently be examined by microscopy or prepared for cellular, molecular, and functional analyses, depending on the research question.
Isolated glomeruli permit direct examination of changes affecting the renal filtration apparatus. Researchers can assess structural findings by microscopy and investigate cellular or molecular alterations associated with glomerular injury, inflammation, and chronic kidney disease. The material may also support functional studies, helping connect observed tissue changes with defects in filtration or responses to treatment.
This approach is useful when investigators need glomerulus-focused evidence rather than measurements from whole kidney tissue. It supports pathology studies, biomarker identification, and research into mechanisms underlying filtration defects. Isolated samples can also help evaluate treatment responses, making the method relevant to studies of glomerular injury, inflammation, and chronic kidney disease.