Multi-angle Rotational Imaging
Multi-angle Rotational Imaging - Related Videos
08:41
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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Cited by 11 •
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Lensfree optical tomography is a three-dimensional microscopy technique that offers a spatial resolution of <1 μm × <1 μm × <3 μm in x, y and z dimensions, respectively, over a large imaging-volume of 15-100 mm3, which can be particularly useful for integration with lab-on-a-chip platforms.
04:19
Size-Exclusion Chromatography with Multi-Angle Light Scattering for Protein Oligomers
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2025
This video describes SEC-MALS, which leverages the abilities of size-exclusion chromatography (SEC) and multi-angle light scattering (MALS). In SEC-MALS, the SEC separates proteins or protein complexes based on their size, and the MALS detector determines the average molecular weight by measuring the intensity of the light scattered by the molecule at different angles.
05:56
Mental Rotation
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Visual mental imagery refers to the ability to conjure images in one’s mind’s eye. This allows people to process visual material above and beyond the constraints of a current point-of-view; for example, a person could imagine, using their mind’s eye, how something might look in a different color, or what it would look like if it were made from a different material or rotated and seen from a different perspective. Mental imagery...
06:31
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
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Cited by 10 •
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The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.
07:48
Rotational Inertia
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2023
Source: Nicholas Timmons, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA Inertia is the resistance of an object to being accelerated. In linear kinematics, this concept is directly related to the mass of an object. The more massive an object, the more force is required to accelerate that object. This is seen directly in Newton's second law, which states that force is equal to mass times acceleration. For rotation,...
FAQs
Controlled rotation samples the anatomy from changing geometric viewpoints, giving reconstruction software multiple projections to relate to one another. That additional angular information helps clarify spatial relationships that may be ambiguous in an individual image. The resulting cross-sectional or three-dimensional representation can therefore show anatomy and structural abnormalities more completely than a single projection.
Multiple projections preserve geometric information from different directions, allowing the reconstructed result to represent how structures are positioned relative to one another. This broader spatial context can support recognition of abnormalities that might be incompletely characterized in one view. The benefit is not simply more images, but a more informative representation of anatomy for medical interpretation.
These factors must be balanced during acquisition. Increasing the amount of imaging may support greater structural detail, while longer scanning can affect scan time and patient exposure. The practical goal is to obtain projections sufficient for useful reconstruction without treating maximum image collection as the only priority. This balance influences the quality and suitability of the final representation.
Acquisition begins by positioning the imaging system or the patient so controlled rotation can occur around the anatomical structure of interest. Sequential images are then recorded at changing angles. Software combines those projections during reconstruction, producing cross-sectional or three-dimensional information for subsequent visualization. Consistent geometric acquisition is important because reconstruction depends on how the views relate to one another.
Its applications include diagnosis, treatment planning, image-guided procedures, and medical research. The method is particularly relevant when understanding anatomy from only one projection is insufficient and spatial relationships influence the clinical or investigative question. Reconstructed information can help users examine structural abnormalities, plan an intervention, or study anatomical organization in a more complete way.
Reconstructed images can provide cross-sectional or three-dimensional views that clarify anatomy, spatial relationships, and structural abnormalities. In treatment planning, this information supports a more complete assessment of the structure involved. During image-guided procedures, the same spatial representation can help relate the intended intervention to surrounding anatomy, while the usefulness of the result depends on the acquisition and reconstruction balance.