Biology
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Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
Chapters
Summary December 12th, 2015
The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.
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