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Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
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Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

English

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8,909 Views

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06:31 min

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December 12, 2015

DOI:

10.3791/53437-v

DOI:
10.3791/53437-v

•

06:31 min
December 12, 2015

•

1 Views
1Graduate School of Frontier Sciences,The University of Tokyo

Summary

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The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.

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Cell Surface DynamicsVariable-angle Epifluorescence MicroscopyVAEMReal-time ImagingPlant Cell BiologyMembrane TraffickingCytoskeletal RearrangementFluorescently-labeled ProteinsPATROL1Stomatal MovementPlasma MembraneArabidopsis ThalianaKymograph AnalysisTime-lapse Imaging

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Cite this Article

Higaki, T. Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy. J. Vis. Exp. (106), e53437, doi:10.3791/53437 (2015).

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