Muscle Volume Measurement
Muscle Volume Measurement - Related Videos
09:15
Measuring Pressure Volume Loops in the Mouse
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Cited by 16 •
2016
This manuscript describes a detailed protocol for the collection of pressure-volume data from the mouse.
08:51
Understanding Concentration and Measuring Volumes
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2023
Solutions are utilized to some degree in almost all biological research applications. Therefore understanding how to measure and manipulate them is imperative to any experiment. In this video, concepts in preparing solutions are introduced. Solutions consist of a solute dissolved in solvent to yield a homogeneous mixture of molecular substances. Solutions are generally identified by their components and corresponding concentrations. Concentrated solutions are diluted through various...
09:49
Measurement of the Pressure-volume Curve in Mouse Lungs
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Cited by 47 •
2015
Here we present a protocol to simply and reliably measure the lung pressure-volume curve in mice, showing that it is sufficiently sensitive to detect phenotypic parenchymal changes in two common lung pathologies, pulmonary fibrosis and emphysema. This metric provides a means to quantify the lung’s structural changes with developing pathology.
03:26
Measuring Compound Muscle Action Potentials in Mouse Forelimb Muscles In Vivo
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2025
This video demonstrates an in vivo technique for measuring compound muscle action potential (CMAP) in mice. It outlines the steps for setting up electrodes, stimulating forelimb neurons that innervate muscle fibers, and recording muscle responses. The technique assesses neuronal demyelination and functional neuron loss.
02:25
C. elegans Muscle Area Measurements: A Standardized Method to Quantify Striated Muscle Morphology
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2023
C. elegans have two types of muscles, single sarcomere and multiple sarcomere, or striated muscles. This video describes the arrangement of the striated body wall muscles, which are responsible for a worm’s locomotion and includes a sample protocol to measure muscle morphology using image analysis software.
FAQs
Image segmentation determines which regions of an anatomical image belong to skeletal muscle rather than surrounding structures. The method then uses those boundaries across sequential slices to calculate the enclosed volume. Consistent separation is essential because including adjacent tissue or omitting muscle can alter the estimate. This makes segmentation a major source of measurement quality and comparability.
Standardization makes measurements from different time points more comparable. Researchers and clinicians need consistent image acquisition, tissue separation, and volume calculation when evaluating disease progression, rehabilitation, exercise, or therapy. Without a stable assessment approach, an apparent change may reflect measurement differences rather than a true alteration in muscle size, weakening clinical monitoring and research conclusions.
A change may provide an objective indicator of muscle wasting, aging-related alterations, rehabilitation progress, or response to exercise or treatment. The measurement contributes information about body composition and can complement assessment of physical function. In neuromuscular disorders and other medical conditions, repeated volume estimates help document structural changes relevant to monitoring and study outcomes.
The workflow begins by acquiring anatomical images, commonly through magnetic resonance imaging or computed tomography. Muscle tissue is then separated from surrounding structures through image segmentation. Finally, the enclosed muscle regions are calculated across sequential slices to estimate total volume. Keeping these stages consistent supports objective comparison between individuals, time points, or treatment conditions.
This assessment is useful when muscle size must be monitored objectively in settings involving muscle wasting, rehabilitation, neuromuscular disorders, aging, exercise, or therapy. It can serve as an outcome measure in research studies and as a monitoring tool in clinical care. Its value is greatest when changes in structure may inform evaluation of disease or treatment.
In medicine, the measurement links anatomical change with clinically relevant questions about physical function, disease, and treatment response. Imaging-based values can strengthen follow-up by providing a quantitative outcome rather than relying only on general observation. They also help research studies evaluate whether rehabilitation, exercise, or therapy is associated with measurable changes in skeletal muscle.