Prostate Tissue Grafting
Prostate Tissue Grafting - Related Videos
11:29
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
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Cited by 16 •
2015
Here we describe a simplified protocol for microRNA (miRNA) expression analyses in archived Formalin-Fixed, Paraffin-Embedded (FFPE) or fresh frozen prostate cancer (PCa) clinical tissues employing quantitative real-time PCR (RT-PCR) and in situ hybridization (ISH).
08:38
Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia
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Cited by 13 •
2013
We describe the manufacture of compressed hormone pellets, as well as subcutaneous surgical implantation into mice. This strategy can be combined with the growth of cell and tissue xenografts under the renal capsule of athymic nude mice to evaluate hormonal carcinogenesis and regulation of benign prostate growth.
02:34
Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate
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2023
This video describes a technique to harvest individual lobes from the prostate gland of a mouse model. Individual lobes can then be used for histological analysis and cancer research.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
04:37
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
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2023
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
FAQs
Host vascularization supplies the transplanted tissue with the living support needed to persist after placement. Because grafts depend on vascularization from the recipient site, the local environment becomes an active part of the experimental system rather than a passive location. This allows researchers to examine tissue organization, growth, and function under conditions shaped by the host.
Signals from the recipient microenvironment help regulate how grafted prostate tissue behaves outside its original location. These signals can affect tissue organization, growth, and function while also shaping interactions among epithelial, stromal, and immune compartments. Studying the graft in this context helps researchers assess how surrounding conditions contribute to development, regeneration, disease progression, or therapeutic response.
Hormone manipulation provides a way to expose androgen-dependent epithelium to changing physiological conditions. Researchers can then observe how epithelial tissue responds when hormonal support is altered, linking hormone sensitivity to changes in organization, growth, or function. This makes grafting useful for investigating androgen-related biology in a living tissue context rather than examining isolated cells alone.
A graft preserves relationships among epithelial, stromal, and immune compartments within living tissue. Researchers can therefore study these components together as they respond to host vascularization, local signaling, and hormonal changes. This integrated setting may reveal effects that are difficult to interpret when each compartment is considered separately, supporting analysis of tissue development, regeneration, disease, and treatment responses.
The approach begins with prostate tissue fragments and their transplantation into a recipient site. After placement, the graft depends on vascularization from the host and continued communication with the local microenvironment. Researchers may then apply hormone manipulation and examine resulting changes in tissue organization, growth, and function, using the living graft to evaluate biological responses over the study.
Researchers use this experimental approach when they need to study prostate biology in living tissue while preserving interactions among multiple compartments. Supported applications include investigating prostate development and regeneration, tracking disease progression, and examining therapeutic response. The method also helps evaluate how androgen-dependent epithelium reacts to altered physiological conditions and how the surrounding tissue environment influences those outcomes.