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Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Bioengineering
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JoVE Journal Bioengineering
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

DOI:
10.3791/52565-v

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13:04 min

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March 18, 2015

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, , , ,
1Cardiovascular Division,King’s College London BHF Centre

Chapters

  • 00:05Title
  • 01:39Decellularized Aorta Graft Preparation
  • 04:31Dual Seeding of PiPSC and Bioreactor Conditioning
  • 08:56Grafting of Double-seeded PiPSC Graft into Mice
  • 11:02Results: Generation of Double-seeded PiPSC-derived Tissue Engineered Vascular Grafts in a Mouse Model
  • 12:17Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.

Tags

Vascular GraftVascular ConduitDecellularized ScaffoldBioreactorInduced Pluripotent Stem CellsEndothelial CellsSmooth Muscle CellsRegenerative MedicineCell Therapy

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