Sap Injection
Sap Injection - Related Videos
04:18
Injecting Self-Assembling Peptides and Neural Precursor Cells for Spinal Cord Repair in a Rat Model
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2025
Source: Zweckberger, K., et al. Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury. J. Vis. Exp. (2015).This protocol demonstrates a targeted injection approach for spinal cord injury repair in a rat model. It involves delivering self-assembling peptides (SAPs) into the lesion core to create a supportive scaffold, followed by neural precursor cell (NPC) injections around the lesion to remodel the tissue.
11:23
Phloem Sap Sampling from Brassica napus for 3D-PAGE of Protein and Ribonucleoprotein Complexes
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Cited by 13 •
2018
Here we present a protocol to analyze the protein composition of large native protein:protein and protein:nucleic acid complexes from oilseed rape (B. napus) phloem exudate using a 3D polyacrylamide gel electrophoresis (PAGE) approach combining blue native (BN) with two denaturing PAGEs followed by mass spectrometric identification.
08:28
Zebrafish Brain Ventricle Injection
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Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
03:48
Mouse Bladder Wall Injection
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Cited by 34 •
2011
Mouse bladder wall injection is a useful approach to orthotopically study bladder stem cell and cancer biology. This delicate microsurgical method can be mastered with careful technique and practice.
FAQs
Selectivity comes from attaching saporin to an antibody or ligand that recognizes a receptor on the intended target cells. The conjugate must bind that surface receptor and be internalized before the toxin can act. This targeting step focuses the ablation on cells bearing the relevant receptor, helping distinguish their contribution from effects caused by surrounding tissue.
Once inside the cell, saporin inactivates ribosomes, the structures responsible for translating genetic information into proteins. Protein synthesis then stops, disrupting the cell's ability to maintain its functions and ultimately causing cell death. This intracellular mechanism explains why receptor binding alone is not sufficient; internalization is a critical part of the ablation process.
The antibody or ligand determines which receptor-bearing cells can be recognized and internalize the toxin. Consequently, the targeting component defines the cell population being removed, while saporin supplies the cell-killing mechanism. Changing that recognition element can therefore alter the biological question from one defined cell group's function to another's, provided the appropriate receptor is present.
Targeted ablation is intended to remove a selected cell population rather than indiscriminately injure all nearby tissue. This distinction allows investigators to examine the contribution of specific cells while limiting the interpretation to the targeted population. In medical research, that separation supports mechanistic analysis of circuits and pathways that would be difficult to resolve after nonspecific tissue damage.
Stereotaxic injection places the saporin preparation into a defined location in an animal model, where it can target the selected neuronal population. The resulting cell ablation creates a model for examining what changes when that population is absent. This approach is especially relevant when researchers need to connect a localized neuronal group with a broader physiological or behavioral function.
Researchers apply this approach to study how selected neuronal populations contribute to neural circuits, pain pathways, autonomic regulation, and behavior. Removing the defined cells provides a way to examine changes associated with their loss and to separate their role from that of neighboring tissue. These outcomes support mechanistic studies of neural function in health and disease.
If removing a defined neuronal population changes a measured function or behavior, the result can indicate that the population contributes to the underlying circuit or pathway. Interpretation depends on the targeted population and its receptor-defined specificity. Because the method is designed to separate selected cells from surrounding tissue, it can clarify functional relationships within complex neural systems.