Vascularized Tumors
Vascularized Tumors - Related Videos
05:51
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
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2023
The present protocol describes a detailed, real-time NIR-II fluorescence imaging operation of a mouse using a NIR-II optics imaging device.
07:26
Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research
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Cited by 9 •
2023
This protocol presents a physiologically relevant tumor-on-a-chip model to perform high-throughput basic and translational human cancer research, advancing drug screening, disease modeling, and personalized medicine approaches with a description of loading, maintenance, and evaluation procedures.
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
03:45
Right Hemihepatectomy by Suprahilar Intrahepatic Transection of the Right Hemipedicle using a Vascular Stapler
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Cited by 1 •
2010
This video describes a right hemihepatectomy with intrahepatic transection of the right hemipedicle leaving the hepatoduodenal ligament completely untouched.
08:18
Engineered Vascularized Muscle Flap
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Cited by 6 •
2016
To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
FAQs
As a tumor enlarges, oxygen shortage can activate angiogenic signaling in nearby tissue. This signaling prompts endothelial cells to proliferate, migrate, and organize into new vessel networks. The resulting supply supports continued tumor expansion, while vessel function also contributes to waste removal. Thus, hypoxia links tumor growth to an active vascular response.
Endothelial cells provide the cellular machinery for the vascular response. After angiogenic signaling, they proliferate and migrate, then contribute to the formation of new vessel networks around the expanding tumor. These vessels change the local tumor environment by delivering oxygen and nutrients and removing waste, making endothelial behavior a central focus in studies of tumor vascularization.
Vascularization affects more than tumor size. Functional vessel networks can sustain expansion beyond the limits imposed by simple diffusion, while the vascular state is also associated with disease progression and treatment response. For medicine, these relationships make tumor blood supply relevant to understanding invasion, interpreting therapeutic effects, and identifying biological features that may serve as vascular biomarkers.
Researchers can assess whether a tumor model develops functional vessels, how those vessels support growth, and how vascular changes alter the tumor microenvironment. Such models are useful for examining oxygen and nutrient delivery, waste removal, and responses to strategies designed to modify tumor blood supply. The goal is to connect vascular behavior with measurable tumor growth and treatment outcomes.
Imaging approaches and vascular biomarkers provide complementary ways to study tumor blood supply. Imaging can help characterize vascular features in a tumor, whereas biomarkers can indicate biological states associated with vascularization. Together, these tools support medical investigation of growth, invasion, and treatment response, and can aid evaluation of approaches that target or alter tumor vasculature.
Vascularized tumor models allow anticancer drugs to be evaluated in a setting that includes tumor-associated vessel networks and the surrounding microenvironment. They can also be used to test strategies intended to alter tumor vascularization or the broader tumor microenvironment. This context helps researchers examine how vascular support relates to tumor growth and treatment response rather than studying tumor cells in isolation.