Cortical Hemisphere Imaging
Cortical Hemisphere Imaging - Related Videos
13:47
Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development
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Cited by 14 •
2013
This protocol describes an improved explant procedure that involves ex utero electroporation, dissection and culture of entire cerebral hemispheres from the embryonic mouse. The preparation facilitates pharmacological studies and assays of gene function during early cortical development.
08:39
Assessing Cortical Cerebral Microinfarcts on High Resolution MR Images
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Cited by 24 •
2015
A high resolution ex vivo 7T MR imaging protocol is presented, to perform MR-guided histopathological validation of microvascular pathology in post-mortem human brain tissue. Furthermore, guidelines are provided for the assessment of cortical microinfarcts on in vivo 7T as well as 3T MR images.
09:57
How to Measure Cortical Folding from MR Images: a Step-by-Step Tutorial to Compute Local Gyrification Index
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Cited by 168 •
2012
Measuring gyrification (cortical folding) at any age represents a window into early brain development. Hence, we previously developed an algorithm to measure local gyrification at thousands of points over the hemisphere1. In this paper, we detail the computation of this local gyrification index.
16:33
Functional Calcium Imaging in Developing Cortical Networks
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Cited by 32 •
2011
Spontaneous activity of developing neuronal networks can be measured using AM-ester forms of calcium-sensitive indicator dyes. Changes in intracellular calcium, indicating neuronal activation, are detected as transient changes in indicator fluorescence with one- or two-photon imaging. This protocol can be adapted for a range of developmentally-dependent neuronal networks in vitro.
09:45
In vivo Imaging of Deep Cortical Layers using a Microprism
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Cited by 11 •
2009
Right-angle microprisms inserted into the mouse neocortex allows for deep imaging of multiple cortical layers with a viewpoint typically found in slice. One-millimeter microprisms offer a wide field-of-view (~900 μm) and spatial resolutions sufficient to resolve dendritic spines. We demonstrate layer V neuronal imaging and neocortical vascular imaging using microprisms.
FAQs
Each imaging modality emphasizes a different property of the hemispheres. Anatomical approaches show tissue structure and organization, whereas functional approaches detect changes associated with blood flow, oxygenation, or electrical and magnetic activity. This distinction matters because an apparent difference between hemispheres may reflect anatomy, momentary activity, or both, depending on the signal being measured.
Direct comparison can reveal lateralization, meaning the preferential involvement of one hemisphere in particular information-processing functions. Researchers examine whether cortical regions show different structure or activity across sides and relate those patterns to behavior or cognition. Such comparisons help distinguish broad hemispheric organization from more localized regional contributions.
Combining the two perspectives links the physical organization of the cortex with its activity during information processing. Anatomical findings can provide structural context for functional patterns, while functional results indicate how regions operate in a given condition. Together, these measurements support more informative interpretations of cortical specialization, connectivity, and behavioral performance than either perspective alone.
A typical workflow selects an imaging approach suited to the question, acquires measurements from the left and right cerebral hemispheres, and compares anatomical or signal-related patterns across regions. Researchers may then relate those observations to behavior, cognitive performance, or connectivity. Using complementary modalities can strengthen interpretation when structure and activity must be considered together.
This approach is useful when investigators need to examine functional specialization, hemispheric lateralization, or relationships between cortical organization and behavior. It also supports studies of development, neurological disease, injury, and treatment. The specific modality depends on whether the investigation emphasizes tissue structure, activity-related signals, connectivity, or changes associated with a condition.
Studies can produce maps of cortical organization, measures for comparing corresponding regions across hemispheres, and evidence about connectivity or functional specialization. When imaging results are related to cognitive performance or behavior, they can help explain how cortical patterns correspond to observed abilities. Longitudinal or clinical comparisons may also characterize changes linked with development, disease, injury, or treatment.