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Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development
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Neuroscience
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JoVE Journal Neuroscience
Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

DOI:
10.3791/50271-v

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13:47 min

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April 03, 2013

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, , ,
1Department of Neuroscience and Physiology,SUNY Upstate Medical University

Chapters

  • 00:05Title
  • 02:00Ex utero Electroporations with GFP
  • 04:04Whole Hemisphere Explant Preparation
  • 06:25Fixation, Embedding, and Sectioning
  • 09:30Neuronal Development in Injected, Cultured, and Processed Hemispheres
  • 13:11Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an improved explant procedure that involves ex utero electroporation, dissection and culture of entire cerebral hemispheres from the embryonic mouse. The preparation facilitates pharmacological studies and assays of gene function during early cortical development.

Tags

Keywords: Cortical DevelopmentCerebral Hemisphere ExplantEx Utero ElectroporationOrganotypic EnvironmentNeuronal MigrationNeuronal DifferentiationLive ImagingPreplate SplittingCortical Layer Formation

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