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Ex utero Electroporation and Whole Hemisphere Explants

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Article DOI: 10.3791/50271 13:47 min
April 3rd, 2013


Summary April 3rd, 2013

This protocol describes an improved explant procedure that involves ex utero electroporation, dissection and culture of entire cerebral hemispheres from the embryonic mouse. The preparation facilitates pharmacological studies and assays of gene function during early cortical development.

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