Lactate Biosensor
Lactate Biosensor - Related Videos
03:08
Colorimetric Assay for Quantification of Lactate in Test Samples
0 Views •
2025
This video describes a colorimetric assay for lactate measurement in cell lysates by using the lactate dehydrogenase enzyme. This enzyme converts lactate into pyruvate, along with the conversion of NAD to NADH that reacts with tetrazolium dyes and gives a colored product that can be measured spectrophotometrically to quantitate the lactate.
01:18
Detection of Human Fecal Metabolites Using a Bacterial Biosensor
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2026
Begin with a bacterial biosensor culture suspended in a medium.These engineered bacteria produce a transcription factor that, upon binding to a specific fecal metabolite, activates a promoter associated with a fluorescent reporter gene.To this culture, add a filtered human fecal sample containing metabolites, such as lactate, that are involved in host-microbiome interactions.Dilute this mixture with a fresh medium to maintain an optimal reaction volume.Incubate without shaking to mimic the...
10:45
Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
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Cited by 20 •
2012
A procedure is described for manipulating the activity of cerebral cortical pyramidal neurons optogenetically while the electroencephalogram, electromyogram, and cerebral lactate concentration are monitored. Experimental recordings are performed on cable-tethered mice while they undergo spontaneous sleep/wake cycles. Optogenetic equipment is assembled in our laboratory; recording equipment is commercially available.
07:30
Using Chronic Social Stress to Model Postpartum Depression in Lactating Rodents
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Cited by 37 •
2013
This article describes the use of chronic resident intruder social stress as an ethologically relevant paradigm to model postpartum depression and anxiety in lactating rodents.
14:04
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Cited by 17 •
2013
Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.
FAQs
Lactate oxidase catalyzes the conversion of lactate to pyruvate and produces hydrogen peroxide as part of that reaction. The hydrogen peroxide is then oxidized at the electrode, generating an electrical current. Because the current varies in proportion to lactate concentration, the signal provides a quantitative readout of metabolic changes in the sampled biological environment.
The electrode current links the enzyme reaction to a measurable electrical output. When lactate oxidase processes more lactate, the associated hydrogen peroxide production changes the oxidation current accordingly. This relationship allows researchers to use current changes to track lactate concentration rather than relying only on endpoint measurements, supporting observation of changing metabolic conditions over time.
High temporal resolution allows extracellular lactate changes to be followed as neural or metabolic conditions change, rather than only measuring a final concentration. This is valuable for examining lactate dynamics during neuronal activity, exercise, hypoxia, or disease. The resulting time-sensitive data can help relate metabolic shifts to changing brain or neural-cell states.
In neuroscience applications, measurements can target extracellular lactate in brain tissue or in neural cell cultures. These settings provide complementary views of lactate-associated metabolism: brain-tissue measurements address changes in an intact neural environment, whereas cultures support investigation of metabolic behavior in neural cells under defined experimental conditions.
By monitoring extracellular lactate, researchers can examine how lactate-associated metabolism changes in neural environments containing neurons and astrocytes. Measurements from brain tissue or neural cell cultures can be compared with conditions involving neuronal activity or other metabolic challenges. This approach supports investigation of lactate's contribution to communication between these cell types and to brain function.
Lactate biosensor measurements can be used to examine metabolic changes associated with neuronal activity, exercise, hypoxia, and disease. Comparing lactate signals across these conditions helps researchers identify how neural metabolism responds to different physiological or pathological contexts. The measurements can also support broader studies of lactate's role in brain function and cellular energy metabolism.