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Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
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Neuroscience
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JoVE Journal Neuroscience
Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

DOI:
10.3791/4328-v

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10:45 min

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December 19, 2012

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, , ,
1Department of Veterinary & Comparative Anatomy, Pharmacology and Physiology, Sleep and Performance Research Center, WWAMI Medical Education Program,Washington State University

Chapters

  • 00:05Title
  • 02:18Anesthesia, Stereotaxic Placement and Clearance of Skull
  • 03:20Cannulas, EMG Leads and EEG Leads
  • 04:55Assembly of Rodent Optogenetic Stimulation System
  • 06:42Measurement of EEG-defined Sleep and Lactate Concentration during Optogenetic Stimulation
  • 08:19Optogenetic Stimulation: EEG-defined Sleep and Lactate Concentration
  • 09:26Conclusion

Summary

Automatic Translation

Automatic Translation

A procedure is described for manipulating the activity of cerebral cortical pyramidal neurons optogenetically while the electroencephalogram, electromyogram, and cerebral lactate concentration are monitored. Experimental recordings are performed on cable-tethered mice while they undergo spontaneous sleep/wake cycles. Optogenetic equipment is assembled in our laboratory; recording equipment is commercially available.

Tags

Simultaneous ElectroencephalographyReal-time MeasurementLactate ConcentrationOptogenetic ManipulationNeuronal ActivityRodent Cerebral CortexGlucose UtilizationNon Rapid Eye Movement SleepCerebral MetabolismSlow WavesElectroencephalogramDepolarized/up StateHyperpolarized/down StateAction Potentials

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