Selex Methodology
Selex Methodology - Related Videos
06:30
SELEX-Based In Vitro Binding Assay to Identify RNA-Protein Interactions
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2025
In this video, we describe the systematic evolution of ligands by the exponential enrichment (SELEX) method to identify specific RNA-binding sequences for a target protein of interest. The protein is incubated with a large pool of randomized RNA sequences, and the protein-binding RNA sequences are isolated, PCR-amplified, and sequenced to identify their protein-binding sites.
01:35
Introduction to New Approach Methodologies (NAMs)
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. New approach methodologies (NAMs) are innovative scientific approaches that generate biological data while reducing reliance on traditional vertebrate animal models. As part of the broader effort to implement the principles of...
07:07
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
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Cited by 2 •
2015
The synthesis of asymmetric species of ferrocene is challenging using solution techniques. This report focuses on the methods carried out to produce a ferrocene-biotin bioconjugate using facile and clean reactions accomplished via solid-phase synthesis. Incorporation of a thiolate moiety is shown to impart the ability for immobilization on gold surfaces.
08:05
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
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Cited by 19 •
2011
Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.
01:16
Response Surface Methodology
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2025
Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable. The process of RSM involves several key steps: Conducting Experiments: The first step is to conduct experiments by systematically varying the input variables. This is typically done using a specific experimental design, such as a factorial...
FAQs
The library supplies many different DNA or RNA sequences, each with the potential to interact with the chosen target. When the library is exposed to that target, sequences with suitable binding behavior are retained while unbound molecules are removed. Amplifying the retained pool preserves those candidates for subsequent rounds, causing favorable binding properties to become more represented over time.
Increasing stringency in later rounds makes it more difficult for weakly or nonspecifically interacting sequences to remain in the enriched pool. This progressive pressure favors aptamers that bind the selected target more effectively and selectively. The approach therefore does more than recover any binding sequence; it helps refine the population toward improved affinity and specificity.
Aptamers provide a nucleic-acid-based alternative to antibodies for recognizing selected molecular targets. Their value in this context comes from the ability to generate DNA or RNA ligands through iterative selection and enrichment rather than relying exclusively on antibody reagents. This expands the available options for detecting neural targets and for developing selective molecular tools.
A typical workflow begins with a randomized DNA or RNA library and a chosen molecular target. The library is exposed to the target, bound and unbound molecules are separated, and the retained sequences are amplified to form the input for another cycle. Repeating these stages, with greater selection stringency when appropriate, enriches the pool for stronger candidates.
In neuroscience, selection can be directed toward neural proteins, receptors, or cell-surface markers. The chosen target determines what kind of molecular recognition the resulting aptamers can provide. Such target-specific ligands support selective detection and can help researchers distinguish or investigate molecular features associated with neural systems.
Aptamers selected against neural targets can support molecular imaging, biomarker analysis, and diagnostic development. Their selective binding can also enable targeted delivery or experimental modulation of neural signaling. These applications make the method relevant both for identifying molecular features in neuroscience and for designing tools that interact with specific neural components.