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Biology

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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
 

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Article DOI: 10.3791/3715
December 12th, 2011

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Summary December 12th, 2011

Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.

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