Orthogonal Projections

Orthogonal projections are mathematical operations that represent a vector or point by its closest component in a specified subspace, making them essential for resolving physical quantities into meaningful directions. Using an inner product, the projection onto a unit direction is found by multiplying that direction by the vector’s dot product with it; subtracting this component leaves a residual perpendicular to the subspace. In physics, these components clarify forces along inclined surfaces, velocities relative to coordinate axes, and electric or magnetic field contributions, while projection operators also provide a foundation for analyzing states in abstract vector spaces.

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Education

JoVE Core - Calculus

Orthogonal Trajectories

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2026

Orthogonal trajectories describe the geometric relationship between two families of curves that intersect each other at right angles. One illustrative case involves a family of parabolas that open sideways along the x-axis. These curves share a common shape but differ by a scaling parameter, resulting in a set of curves that all pass through the origin and widen at different rates.Determining Orthogonal TrajectoriesTo identify the orthogonal trajectories for these parabolas, the first step...

Research

JoVE Journal - Biology
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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

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Cited by 7 •

2012

A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.

Calibration Procedures for Orthogonal Superposition Rheology

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Cited by 4 •

2020

We present a detailed calibration protocol for a commercial orthogonal superposition rheology technique using Newtonian fluids including end-effect correction factor determination methods and recommendations for best practices to reduce experimental error.

Fischer Projections

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2023

Learning to draw Fischer projections of molecules and understanding their relevance plays a crucial role in the visual depiction of organic molecules. A Fischer projection is a two-dimensional projection on a planar surface to simplify the three-dimensional wedge–dash representation of molecules. This is especially helpful in the case of molecules with multiple chiral centers that can be difficult to draw. Here, all the bonds of interest are represented as horizontal or vertical lines. While...

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

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Cited by 15 •

2009

We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.

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