Mann-whitney U Test

The Mann-Whitney U test is a nonparametric statistical method for comparing two independent groups when data may not meet the assumptions required by a t-test. It combines observations from both groups, ranks them from smallest to largest, and evaluates whether one group tends to have higher or lower values than the other by calculating the U statistic and its associated significance. Researchers use the test for ordinal or continuous measurements, particularly with skewed data or small samples, to assess differences in distributions, central tendency, or group outcomes across biomedical, behavioral, and experimental studies.

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JoVE Journal - Biochemistry

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

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2020

This article describes the experimental procedures for (a) depletion of U1 snRNP from nuclear extracts, with concomitant loss of splicing activity; and (b) reconstitution of splicing activity in the U1-depleted extract by galectin-3 - U1 snRNP particles bound to beads covalently coupled with anti-galectin-3 antibodies.

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2025

We present calcium imaging protocols for a Drosophila larval olfactory neuron, using a topical tissue adhesive for immobilization. This method enhances stability, facilitating reliable in-vivo and ex-vivo experiments. Custom R scripts analyze calcium signals, providing an efficient platform for detailed neurophysiological research.

Machine Learning-Based Cough Tone Classification: Diagnostic Exploration of Chronic Obstructive Pulmonary Disease and Respiratory Tract Infections

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2025

This study effectively accomplished the automated classification of two distinct categories by acquiring cough sound data from patients diagnosed with chronic obstructive pulmonary disease (COPD) and respiratory tract infections (RTI), utilizing an integration of speech signal processing techniques and machine learning algorithms.

Midface Hypoplasia and Cranial Base Morphology in Syndromic Craniosynostosis: A Comparative Analysis Study Using a Predictive Regression Model

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2025

This study proposed an age-adjusted regression modeling using midface and cranial base morphology as a potential tool for preoperative evaluation and individualized surgical planning for children with syndromic craniosynostosis (SC).

Single Oocyte Bisulfite Mutagenesis

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Cited by 14 •

2012

Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.

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