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June 27, 2012
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Summary
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Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.
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Cite this Article
Denomme, M. M., Zhang, L., Mann, M. R. Single Oocyte Bisulfite Mutagenesis. J. Vis. Exp. (64), e4046, doi:10.3791/4046 (2012).
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