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Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo
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JoVE Journal Biologie
Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

DOI:
10.3791/1382-v

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09:25 min

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September 15, 2009

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,
1Department of Molecular and Cellular Biology,University of California, Davis

Kapitel

  • 00:00Titel
  • 00:48Introduction
  • 01:30Lay Cage and Needle Preparation
  • 03:07Coverslip and Embryo Preparation
  • 05:58Injecting and Imaging the Embryos
  • 07:26Representative Embryo Injection Results
  • 08:43Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol describes the use of microinjection and high resolution imaging in the Drosophila melanogaster syncytial embryo to study mitosis.

Tags

Microinjection TechniquesStudying MitosisDrosophila Melanogaster Syncytial EmbryoGenetikTransgenic FliesFluorescently Tagged Mitotic ProteinsGAL4/UAS SystemCell Cycle DurationImaging MitosisRapidly Dividing NucleiMitotic MachineryCheckpointLive DynamicsFunction-blocking AntibodiesProtein Inhibitors

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