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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Article DOI: 10.3791/162-v • 22:07 min • February 25th, 2007
February 25th, 2007 •
1, 1
1Childrens Hospital, Harvard Stem Cell Institute, Harvard Medical School

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This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.

Tags

Stem Cells Hematopoietic Stem Cells Murine Embryonic Stem Cells Self-renewal Differentiated Progeny Pluripotent Differentiative Ability Clinically Useful Cell Types Video Protocol Hematopoietic Malignancies Hematopoietic Disorders HSC Therapy Alternative Source Of HSC Baseline Human ESC Isolation Embryoid Bodies (EBs) Serum Pre-screened Hematopoietic Differentiation Retroviral HoxB4 OP9 Stroma Bone Marrow Stromal Cell Line Cytokines
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