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Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
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Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Article DOI: 10.3791/1963-v • 10:39 min • June 12th, 2010
June 12th, 2010 •
1, 1
1Dept. Of Cell Biology and Molecular Genetics, University of Maryland

Kapitel

Summary
Automatic Translation

This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

Tags

Bimolecular Fluorescence Complementation BiFC Assay Protein-protein Interaction Onion Cells Helios Gene Gun Gene Function Fluorescent Protein GFP Plant Researchers Onion Epidermal Cells Plasmid DNA Arabidopsis Thaliana SEUSS LEUNIG HOMOLOG
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