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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
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Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)

Article DOI: 10.3791/2078-v • 07:02 min • September 14th, 2010
September 14th, 2010 •
1, 1
1Department of Microbiology & Immunology and the Center for AIDS Health Disparities Research, Meharry Medical College

Kapitel

Summary
Automatic Translation

Host cell factors play a critical role in the establishment and maintenance of Kaposi's sarcoma (KS). We outline methods to identify host cell factors altered in KSHV-infected DMVEC cells, and in KS tumor tissue. Cellular genes altered by virus will serve as potential target(s) for novel therapeutics.

Tags

Kaposi's Sarcoma-associated Herpesvirus Dysregulated Genes KSHV HIV/AIDS Malignancy Southern Africa Angioproliferative Tumor Vascular Endothelial Cells B Cell Lymphoproliferative Diseases Pleural Effusion Lymphomas Multicentric Castleman's Disease Lytic Replication Oncogenesis Tumor Progression Host-factor Determinants Prognostic Markers Metastasis Therapeutics Dermal Microvascular Endothelial Cells (DMVEC) Microarray Analysis Protein Expression Immunoblot Dual Labeled Immunofluorescence Transcriptional Expression Quantitative Real-time Polymerase Chain Reaction (qRT-PCR)
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