Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization

doi:10.3791/21131

Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization

Encyclopedia of Experiments
Biological Techniques

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Encyclopedia of Experiments Biological Techniques

Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization

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For fluorescence-detected size-exclusion chromatography or FSEC analysis, next, equilibrate a size-exclusion column with a broad fractionation range with running flow buffer at a rate of 0.3 milliliters per minute. Then, inject 100 microliters of a solubilized membrane sample onto the column at the same flow rate, and monitor the illusion profile using fluorescence optics.

Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization

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