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Immunology and Infection

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Peptide: MHC Tetramero basata Arricchimento di epitopi specifici per cellule T

Article DOI: 10.3791/4420-v • 13:58 min • October 22nd, 2012
October 22nd, 2012 •
1, 1
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School

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Questo protocollo descrive l'uso del peptide: MHC tetrameri e microsfere magnetiche per isolare popolazioni bassa frequenza di epitopo-specifiche cellule T e analizzarli mediante citometria a flusso. Questo metodo consente lo studio diretto delle popolazioni di cellule T endogene di interesse da

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Immunologia Numero 68 Biologia Cellulare Biologia Molecolare cellule T recettore delle cellule T tetramero citometria a flusso antigene-specifica l'immunologia la risposta immunitaria magnetico arricchimento,
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