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Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia
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August 08, 2013
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An easy and reliable technique for visualizing and quantifying airway cilia motility and cilia generated flow using mouse trachea is described. This technique can be modified to determine how a wide range of factors influence cilia motility, including pharmacological agents, genetic factors, environmental exposures, and/or mechanical factors such as mucus load.
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Francis, R., Lo, C. Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia. J. Vis. Exp. (78), e50343, doi:10.3791/50343 (2013).
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