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Culturing Microglia from the Neonatal and Adult Central Nervous System
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Culturing Microglia from the Neonatal and Adult Central Nervous System

Culturing Microglia from the Neonatal and Adult Central Nervous System

27,251 Views

11:28 min

August 09, 2013

DOI:

11:28 min
August 09, 2013

28 Views
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Summary

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We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.

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