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Den ChroP Approach Kombinerer Chip og massespektrometri å dissekere Locus-spesifikke proteomikk Landscapes of Kromatin

Article DOI: 10.3791/51220-v • 24:02 min • April 11th, 2014
April 11th, 2014 •
1, 1
1Department of Experimental Oncology, European Institute of Oncology

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Ved å kombinere mors og kryssbinding kromatin immunoprecipitation med høy oppløsning massespektrometri, ChroP tilnærming gjør det mulig å dissekere den sammensatte proteomikk arkitektur histonmodifikasjonene, varianter og ikke-histonic proteiner synergizing på funksjonelt distinkte kromatin domener.

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Biokjemi kromatin histone posttranslasjonelle modifikasjoner (hPTMs) epigenetikk massespektrometri proteomikk SILAC kromatin immunoprecipitation histone varianter chromatome hPTMs kryss-forhandlingene
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