Immundetektion von Außenmembranproteinen mittels Durchflusszytometrie von isolierten Mitochondrien
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September 18, 2014
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Beschrieben wird hier ein Verfahren zur Detektion und Quantifizierung der mitochondrialen Außenmembran-Proteine durch Immunmarkierung von Mitochondrien aus Nagetiergewebe und Analyse durch Durchflusszytometrie Strom isoliert. Diese Methode kann erweitert werden, um funktionale Aspekte der mitochondrialen Subpopulationen zu bewerten.
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Pickles, S., Arbour, N., Vande Velde, C. Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria. J. Vis. Exp. (91), e51887, doi:10.3791/51887 (2014).
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