Verfahren zum Screening und Validierung von Beständig Mutationen Vor-Inhibitoren
14,859 Views
•
12:40 min
•
December 07, 2014
DOI:
Summary
Automatically generated
Entstehung der genetischen Resistenz gegen Kinase-Hemmer-Therapie stellt große Herausforderung für eine effektive Krebstherapie. Identifizierung und Charakterisierung von Mutationen resistent gegen ein neu entwickeltes Medikament hilft bei der besseren klinischen Behandlung und der nächsten Generation Drug-Design. Hier beschreiben wir unser Protokoll für in-vitro-Screening und Validierung von resistenten Mutationen.
Verwandte Videos
09:38
Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure
Verwandte Videos
8791 Views
07:42
Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
Verwandte Videos
7928 Views
10:09
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
Verwandte Videos
8240 Views
07:49
Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
Verwandte Videos
5996 Views
13:22
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
Verwandte Videos
7863 Views
10:49
Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia
Verwandte Videos
18125 Views
14:51
Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype
Verwandte Videos
3180 Views
09:33
Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor
Verwandte Videos
1091 Views
12:40
A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors
Verwandte Videos
14.9K Views
14:57
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Verwandte Videos
14.4K Views
Read Article
Cite this Article
Kesarwani, M., Huber, E., Kincaid, Z., Azam, M. A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors. J. Vis. Exp. (94), e51984, doi:10.3791/51984 (2014).
Copy