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JoVE Journal Bioengineering
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Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Article DOI: 10.3791/52224-v • 11:01 min • September 25th, 2016
September 25th, 2016 •
1, 2, 2, 2, 3, 2, 2, 2, 4, 2
1Radiology, University of Minnesota, 2Medicine, University of Minnesota, 3School of Public Health, University of Minnesota, 4Surgery, University of Arizona

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Summary
Automatic Translation

Nutrient regulation using continuous growth adjusted feeding improves growth rates of mammalian cell spheroids compared to intermittent batch feeding for cultures in stirred suspension bioreactors. This study demonstrates the methods required for establishing simple adjusted rate fed cultures.

Tags

Nutrient Regulation Continuous Feeding Large-scale Expansion Mammalian Cells Spheroids Cell Culture Controlled Nutrient Conditions Algorithms Large Scale Cell Culture Applications Human Cellular Therapies Uniform Distribution Of Nutrients And Gases Higher Cell Densities Static Or Bulk Fed Cell Cultures Yield Cell Loss Equipment Visual Demonstration Method Primary Components Feeding System Medium Reservoir Peristaltic Pump Stirred Bioreactor Waste Reservoir Custom Designed Tubing Sampling Set
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