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Immunologie und Infektion
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Parvise Vekst Konkurranse analyse for Bestemme Replication Fitness av humant immunsviktvirus
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Parvise Vekst Konkurranse analyse for Bestemme Replication Fitness av humant immunsviktvirus

DOI:
10.3791/52610-v

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11:19 min

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May 04, 2015

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, , , , , , , , , , ,
1Department of Microbiology,University of Washington, 2Departments of Medicine and Laboratory Medicine,University of Washington, 3U.S Military HIV Research Program,Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

Kapitel

  • 00:05Titel
  • 01:23Growth Competition
  • 05:00Determination of Viral Ratios
  • 09:16Results: HIV Replication Fitness Determined by Pairwise Growth Competition Assays
  • 10:54Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

Tags

HIV-1Viral Replication FitnessIn Vitro Fitness AssayGrowth Competition AssayMultiplicity Of Infection (MOI)Viral DetectionRelative Fitness Calculation

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