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In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology
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1Interdisciplinary Graduate School,Nanyang Technological University, 2Singapore Centre on Environmental Life Sciences Engineering,Nanyang Technological University, 3School of Biological Sciences,Nanyang Technological University, 4Centre for Marine Bio-Innovation,University of New South Wales, 5School of Biological, Earth and Environmental Sciences,University of New South Wales, 6School of Biotechnology and Biomolecular Sciences Sciences,University of New South Wales
Kapitel
- 00:05Titel
- 00:43Flow Cell or Static Culture Setup
- 01:55Microscopy
- 03:28Particle Tracking Analysis
- 06:11Particle Trajectory Analysis
- 10:01Results: Particle Tracking in Biofilms
- 11:52Conclusion
Particle-tracking microrheology investigates the viscoelasticity of materials. Here, the technique is used to determine the viscoelasticity, creep compliance and effective crosslinking roles of different matrix components of a bacterial biofilm. The matrix consists of polymeric substances secreted by the bacteria and its components determine biofilm structure and mechanical properties.
