Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Mathieu Daynac; Lise Morizur; Thierry Kortulewski; Laurent R. Gauthier; Martial Ruat; Marc-Andr&#233; Mouthon; Fran&#231;ois D. Boussin

doi:10.3791/53247

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Zellsortierung von Neural Stamm- und Vorläuferzellen aus dem erwachsenen Maus Subventrikularzone und Live-Bildgebung ihrer Zellzyklus Dynamics

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

JoVE Journal
Neurowissenschaften

This content is Free Access.

JoVE Journal Neurowissenschaften

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Zellsortierung von Neural Stamm- und Vorläuferzellen aus dem erwachsenen Maus Subventrikularzone und Live-Bildgebung ihrer Zellzyklus Dynamics

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14,148 Views

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09:27 min

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September 14, 2015

DOI:

10.3791/53247-v

DOI:

10.3791/53247-v

•

09:27 min

September 14, 2015

•

14 Views

Mathieu Daynac*^1,2,3,4,5, Lise Morizur*^1,2,3,4, Thierry Kortulewski^1,2,3,4, Laurent R. Gauthier^1,2,3,4, Martial Ruat⁵, Marc-André Mouthon^1,2,3,4, François D. Boussin^1,2,3,4

¹CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, ²INSERM, UMR 967, ³Université Paris Diderot, Sorbonne Paris Cité, UMR 967, ⁴Université Paris Sud, UMR 967, ⁵CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department

Summary

We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.