Lasergestützte Mikrodissektion (LAM) als Werkzeug für die Transkriptionsprofilierung einzelner Zelltypen
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May 10, 2016
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Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.
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Florez Rueda, A. M., Grossniklaus, U., Schmidt, A. Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types. J. Vis. Exp. (111), e53916, doi:10.3791/53916 (2016).
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