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JoVE Journal
Biology

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Measuring Lactase Enzymatic Activity in the Teaching Lab
 

Measuring Lactase Enzymatic Activity in the Teaching Lab

Article DOI: 10.3791/54377-v 04:41 min August 6th, 2018
August 6th, 2018

Kapitel

Summary

The enzymatic activity of lactase is essential for the catabolic processing of the disaccharide lactose. Here, the activity of lactase found in dietary supplements is assayed using a colorimetric assay. This provides students with an experimental platform for understanding the activity of lactase and enzyme kinetics.

Tags

Keywords: Lactase Enzyme Activity Teaching Lab ONPG PBS Spectrophotometry Temperature Enzyme Kinetics
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