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Developmental Biology

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JoVE Journal Developmental Biology
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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Article DOI: 10.3791/54598-v • 08:24 min • February 28th, 2017
February 28th, 2017 •
1, 1, 1, 2, 2, 3, 1
1Stem Cell and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Citta della Speranza, 2Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 3Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital

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Summary
Automatic Translation

In this study, we generate induced pluripotent stem cells from mouse amniotic fluid cells, using a non-viral-based transposon system.

Tags

Pluripotent Stem Cells Mouse Amniotic Fluid Cells Transposon System Reprogramming Therapeutic Setting Human Disease Fetuses Drug Testing Tissue Engineering Patient-specific Therapy Programming Clinical Approach Viral Vectors Oxino-free IPS Cells Isolation Abdominal Wall Laparotomy Forceps Uterus Fetal Membranes Placenta
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