Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Analisi per misurare il trasporto…
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
Analisi per misurare il trasporto nucleocitoplasmatico in tempo reale all'interno di motori neuronici come le cellule NSC-34
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
Journal (Neuroscience)
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice
Journal (Neuroscience)
Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice
 
Click here for the English version

Analisi per misurare il trasporto nucleocitoplasmatico in tempo reale all'interno di motori neuronici come le cellule NSC-34

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Questo protocollo descrive un metodo per misurare sensibilmente il tasso di trasporto nucleocitoplasmatico nelle cellule NSC-34 come motori neuronali quantificando il cambiamento in tempo reale nell'importazione nucleare di una proteina NLS-NES-GFP.

Tags

Neuroscienza Edizione 123 trasporto nucleocitoplasmatico shuttle GFP cellule NSC-34 C9orf72 ALS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter