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Journal / Genetics / In Vitro Mareld Assay att karakterisera…
JoVE Journal
Genetics

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In Vitro Mareld Assay att karakterisera dygnsrytmen i mjölkkörtlar epitelceller
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In Vitro Mareld Assay att karakterisera dygnsrytmen i mjölkkörtlar epitelceller

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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En in vitro- Mareld analys att bestämma cellulära dygnsrytmen i mjölkkörtlar epitelceller presenteras. Denna metod använder tredjeparts däggdjursceller reporter plasmider uttrycker destabiliserat luciferas under kontroll av PERIOD 2 gen arrangören. Det kan anpassas till andra celltyper att utvärdera organ-specifika effekter på dygnsrytmen.

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Genetik problemet 127 Circadian rytm PERIOD 2 promotorn aktivitet, in vitro- realtid Mareld assay luciferas uttryck vektor destabiliserat firefly luciferas plasmid transfection.
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