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Quadruple Immunostaining av Olfactory Bulb for Visualisering av Olfactory Sensory Axon Molecular Identity Codes

Article DOI: 10.3791/55893-v • 06:32 min • June 5th, 2017
June 5th, 2017 •
1, 1,2, 1,3
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST)

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Olfaktoriske sensoriske nevroner uttrykker et bredt utvalg av akson-sorteringsmolekyler for å etablere riktig neural kretsløp. Denne protokollen beskriver en immunhistokjemisk fargemetode for å visualisere kombinatoriske uttrykk for akson-sorteringsmolekyler ved axonterminalene til olfaktoriske sensoriske nevroner.

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Neurovitenskap utgave 124 olfaktorisk system immunhistokjemi genuttrykk axonveiledning neural kretsdannelse olfaktoriske sensoriske nevroner
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