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Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Article DOI: 10.3791/56530-v • 10:39 min • February 19th, 2018
February 19th, 2018 •
*1, *1,2, 1,2, 1,2,3, 1,2, 4
1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Mechanical Engineering, Stanford University, 3Department of Bioengineering, Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology, The Institute of Photonic Sciences (ICFO)
* These authors contributed equally

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Summary
Automatic Translation

New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.

Tags

Microfluidics Device Mechanical Stimulation High Resolution Imaging C. Elegans Pressure Actuators Mechanobiology Sensory Biology Worm's Mobility Non-invasive Worm's Cuticle Mechanical Cues Development Microscope System Excitation Of GCaMP And RFP Discreet Light Source Cyan And Yellow Light 10-times Objective High-magnification Objective Digital Camera Fluorescence Cube Excitation Filter Dichroic Mirror Beam Splitter Long-pass Dichroic Mirror Cutoff At 570 Nanometers Band-pass Emission Filter
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