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Journal / Genetics / Bir bitiş homoloji-aracılı katılmadan tabanlı…
JoVE Journal
Genetics

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JoVE Journal Genetics
Bir bitiş homoloji-aracılı katılmadan tabanlı strateji kullanarak hedeflenen Integration CRISPR/Cas9-aracılı Vivo içinde
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Bir bitiş homoloji-aracılı katılmadan tabanlı strateji kullanarak hedeflenen Integration CRISPR/Cas9-aracılı Vivo içinde

Article DOI: 10.3791/56844-v • 08:22 min • March 12th, 2018
March 12th, 2018 •
1,2, 1,2, 2,3,4, 1, 3, 1
1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Kümelenmiş düzenli olarak kısa palindromik tekrarlar/ilişkili CRISPR protein 9 (CRISPR/Cas9) interspaced sistem genetik mühendisliği için umut verici bir araçtır ve hedeflenen entegrasyonu transgenes olasılığı kadar açılır. Biz (HMEJ) katılmadan homoloji-aracılı son tarif-verimli DNA Tümleştirme vivo içinde hedef ve gen tedavileri CRISPR/Cas9 kullanarak hedef için strateji tabanlı.

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Genetik sayı: 133 CRISPR/Cas9 hedeflenen Tümleştirme homoloji-aracılı son katılma In Vivo embriyo genetik fareler hidrodinamik enjeksiyon modifiye
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