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Journal / Bioengineering / Gel-seq: A Method for Simultaneous Sequencing…
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Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Article DOI: 10.3791/57315-v • 09:19 min • March 26th, 2018
March 26th, 2018 •
*1, *2, 2, 3, 2
1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego
* These authors contributed equally

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Summary
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Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.

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Gel-seq Simultaneous Sequencing Library Preparation DNA RNA Hydrogel Matrices Structural Variations Gene Expression Genomic Information Transcriptomic Information Upright Cassettes Gel Electrophoresis Cassette Filler High Density Precursor Mixture Low Density Precursor Mixture Reagents APS Temed Degas Bubbles Ultra Sonic Bath
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