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Journal / Biochemistry / 线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定…
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Biochemistry

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JoVE Journal Biochemistry
线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定
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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

Article DOI: 10.3791/57807-v • 09:35 min • October 15th, 2018
October 15th, 2018 •
1,2, 3, 3
1Department of Health Science, School of Sports & Health Science, Daito Bunka University, 2Department of Molecular Life Science, School of Medicine, Tokai University, 3Department of Health Management, School of Health Study, Tokai University

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本文介绍了一种改良的小尺度萃取和比色法测定乳酸和丙酮酸在线虫中的含量.使用商用试剂盒时, 其灵敏度和准确度的技术发展非常重要。在萃取中蛋白质沉淀是定量测定细胞内代谢产物的关键步骤。

Tags

生物化学 140 期 线虫线虫 能量代谢 乳酸 丙酮酸 常见沉淀剂 比色测定
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